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KMID : 0903519820250010055
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1982 Volume.25 No. 1 p.55 ~ p.61
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Electrophoretic Studies on Isozymes of Sesamum indicum L .
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Abstract
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The total protein, peroxidase and isocitrate lyase of ungerminated seeds and germinated seedlings of Sesamum indicum L. were studied by disc polyacrylamide get electrophoresis.
In the electrophoretic studies of ungerminated seed proteins, more than 13 bands were observed by amido black 10B staining. Among 13 protein hands, 4 bands showed peroxidase activity and 4 bands isocitrate lyase activity.
During germination it was noted that there were some changes in protein levels within the bands. Proteins with low electrophoretic mobilities increased within 19 hours and decreased after 45 hours, while those with high mobilities remained fairly constant. Protein band sharpness decreased with germination..
During germination the number of peroxidase isozymes increased from 4 to 5 within 45 hours and to 6 in 97 hours. The enzyme activity of each band (except for one hand between 65 and 97 hours) increased with germination and was more apparent after 45 hours.
The number of isozymes in isocitrate lyase did not appear to change during germination. The enzyme activity of each bard decreased with germination and disappeared after 45 hours.
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